[CAS NO. 83-49-8] Hyodeoxycholic acid
PRODUCTS SPECIFICATIONS [83-49-8]
DESCRIPTION [83-49-8]
Overview
| MDL | - |
|---|---|
| Molecular Weight | 392.57 |
| Molecular Formula | C24H40O4 |
| SMILES | C[C@H](CCC(O)=O)[C@H]1CC[C@@]2([H])[C@]3([H])C[C@H](O)[C@]4([H])C[C@H](O)CC[C@]4(C)[C@@]3([H])CC[C@]12C |
Summary
Hyodeoxycholic acid is a secondary bile acid formed in the small intestine by the gut flora, and acts as a TGR5 (GPCR19) agonist, with an EC 50 of 31.6 µM in CHO cells.
IC50 & Target
|
Human Endogenous Metabolite |
In Vitro
Hyodeoxycholic acid is a secondary hydrophilic bile acid formed in the small intestine by the gut flora, and acts as an agonist of TGR5, with an EC 50 of 31.6 µM in CHO cells [1] . Hyodeoxycholic acid (50, 100 μM) increases the expression of genes ( Abca1, Abcg1, and Apoe ) involved in cholesterol efflux in RAW 264.7 cells [2] .
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
In Vivo
Hyodeoxycholic acid (HDCA; 1.25% (wt/wt)) obviously decreases fat mass and increases lean mass but does not raise the serum levels of any organ toxicity markers in LDLRKO mice. Hyodeoxycholic acid inhibits atherosclerotic lesion formation in LDLRKO at multiple sites, improves plasma lipoprotein profiles, decreases plasma glucose level and intestinal cholesterol absorption efficiency and increases daily cholesterol excretion through fecal output. Hyodeoxycholic acid also improves HDL function as measured by a cholesterol efflux assay [2] .
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Appearance
Solid
Shipping
Room temperature in continental US; may vary elsewhere.
Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvent & Solubility
DMSO : ≥ 100 mg/mL ( 254.73 mM )
H 2 O : < 0.1 mg/mL (insoluble)
* "≥" means soluble, but saturation unknown.
Stock Solutions
| Concentration Solvent Mass | 1 mg | 5 mg | 10 mg |
|---|
| 1 mM | 2.5473 mL | 12.7366 mL | 25.4732 mL |
| 5 mM | 0.5095 mL | 2.5473 mL | 5.0946 mL |
| 10 mM | 0.2547 mL | 1.2737 mL | 2.5473 mL |
References
- [1] Sato H, et al. Novel potent and selective bile acid derivatives as TGR5 agonists: biological screening, structure-activity relationships, and molecular modeling studies. J Med Chem. 2008 Mar 27;51(6):1831-41.
- [2] Shih DM, et al. Hyodeoxycholic acid improves HDL function and inhibits atherosclerotic lesion formation in LDLR-knockout mice. FASEB J. 2013 Sep;27(9):3805-17.
Synonyms