[CAS NO. 848035-21-2] Compound48/80trihydrochloride
PRODUCTS SPECIFICATIONS [848035-21-2]
DESCRIPTION [848035-21-2]
Overview
| MDL | - |
|---|---|
| Molecular Weight | 629.10 |
| Molecular Formula | C32H48Cl3N3O3 |
| SMILES | CNCCC1=CC(CC2=CC(CCNC)=CC=C2OC)=C(OC)C(CC3=CC(CCNC)=CC=C3OC)=C1.[H]Cl.[H]Cl.[H]Cl |
Summary
Compound 48/80 trihydrochloride (C48/80 trihydrochloride) is a mixture of condensation products of N-methyl- p -methoxyphenethylamine with formaldehyde. Compound 48/80 trihydrochloride is also a histamine releaser and a mast cell degranulator. Compound 48/80 inhibits phosphatidylinositol-specific phospholipase C activity from human platelets [1] [2] [3] .
In Vitro
Compound 48/80 trihydrochloride (C48/80 trihydrochloride) inhibits both cytosolic and particulate phosphatidylinositol-specific phospholipase C activities with a similar efficiency; IC 50 values are 2.1 μg/ml (supernatant) and 5.0 μg /ml (particulate fraction). The aggregation of human platelets induced by ADP and PAF-acether is inhibited by Compound 48/80 [1] .
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
In Vivo
Compound 48/80 trihydrochloride (0.75 mg/kg; i.p.; Killed after 0.5, 3 or 6 h) increases serum serotonin, histamine and corticosterone levels at 0.5 h, but their increases were reduced thereafter
[3]
.
Compound 48/80 trihydrochloride causes oxidative stress in rat adrenal gland through mast cell degranulation
[3]
.
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
| Animal Model: | 7-week-old Wistar male rats (fasted for 24 hours) [3] |
| Dosage: | 0.75 mg/kg |
| Administration: | Intraperitoneal injection; Killed after 0.5, 3 or 6 h |
| Result: | Serum histamine and serotonin concentrations significantly higher than those in untreated control rats at 0.5 h after treatment. The increased serum histamine and serotonin concentrations in rats decreased time-dependently thereafter. |
Appearance
Solid
Shipping
Room temperature in continental US; may vary elsewhere.
Storage
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvent & Solubility
H 2 O : 25 mg/mL ( 39.74 mM ; Need ultrasonic)
DMSO : 10 mg/mL ( 15.90 mM ; ultrasonic and warming and heat to 60°C)
Stock Solutions
| Concentration Solvent Mass | 1 mg | 5 mg | 10 mg |
|---|
| 1 mM | 1.5896 mL | 7.9479 mL | 15.8957 mL |
| 5 mM | 0.3179 mL | 1.5896 mL | 3.1791 mL |
| 10 mM | 0.1590 mL | 0.7948 mL | 1.5896 mL |
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1.
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2.
Add each solvent one by one: 10% DMSO >> 90% (20% SBE-β-CD in saline)
Solubility: ≥ 2.5 mg/mL (3.97 mM); Clear solution
References
- [1] Bronner C, et al. Compound 48/80 is a potent inhibitor of phospholipase C and a dual modulator of phospholipase A2 from human platelet. Biochim Biophys Acta. 1987 Aug 15;920(3):301-5.
- [2] Schemann M, et al. The mast cell degranulator compound 48/80 directly activates neurons. PLoS One. 2012;7(12):e52104.
- [3] Kaida S, et al. Compound 48/80 causes oxidative stress in the adrenal gland of rats through mast cell degranulation. Free Radic Res. 2010 Feb;44(2):171-80.