[CAS NO. 84929-27-1] Grape seed extract
PRODUCTS SPECIFICATIONS [84929-27-1]
DESCRIPTION [84929-27-1]
Overview
| MDL | - |
|---|---|
| Molecular Weight | - |
| Molecular Formula | Unspecified |
| SMILES | [Grape seed extract] |
Summary
In Vitro
Grape seed extract (25-100 μg/mL; 12-48 hours) inhibits growth and induces death in human colorectal cancer cells
[3]
.
Grape seed extract (25-100 μg/mL; 12-24 hours) modulates the protein levels of cell cycle regulatory molecules in HT29 and LoVo cells
[3]
.
Grape seed extract (25-100 μg/mL; 24 hours) causes apoptotic cell death of human colorectal cancer cells
[3]
.
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Cell Proliferation Assay [3]
| Cell Line: | HT29 cells, LoVo cells |
| Concentration: | 25 μg/mL, 50 μg/mL, 100 μg/mL |
| Incubation Time: | 12 hours, 24 hours, 48 hours |
| Result: | Inhibited cell growth in a dose- and a time-dependent manner. |
Cell Cycle Analysis [3]
| Cell Line: | HT29 cells, LoVo cells |
| Concentration: | 25 μg/mL, 50 μg/mL, 100 μg/mL |
| Incubation Time: | 12 hours, 24 hours, 48 hours |
| Result: | Induced cell cycle arrest (a marked increase in Cip1/p21protein level and a decrease in G 1 phase^associated cyclins and cyclin-dependentkinases). |
Western Blot Analysis [3]
| Cell Line: | HT29 cells, LoVo cells |
| Concentration: | 25 μg/mL, 50 μg/mL, 100 μg/mL |
| Incubation Time: | 12 hours, 24 hours |
| Result: | Modulates the protein levels of CDKIs, CDKs, and cyclins. |
Apoptosis Analysis [3]
| Cell Line: | HT29 cells, LoVo cells |
| Concentration: | 25 μg/mL, 50 μg/mL, 100 μg/mL |
| Incubation Time: | 24 hours |
| Result: | Showed a significant dose-dependent increase in apoptotic cell population in HT29 cells at 50 μg/mL and 100 μg/mL; all three Concentrations caused a dose-dependent increase in apoptotic cells. |
In Vivo
Grape seed extract (200 mg/kg; i.g.; 5 days/wk; for 8 weeks) inhibits HT29 colon carcinoma tumor xenograft growth in athymic nude mice
[3]
.
Grape seed extract inhibits cell proliferation but increases apoptotic cell death in tumors
[3]
.
Grape seed extract enhances Cip1/p21protein levels and poly(ADP-ribose) poly-merase cleavage in tumors
[3]
.
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
| Animal Model: | Athymic male nu/nu mice, with human colon carcinoma HT29 tumor xenograft [3] |
| Dosage: | 200 mg/kg |
| Administration: | Oral gavage, 5 days/wk, for 8 weeks |
| Result: | Showed time-dependent inhibition of tumor growth. |
Clinical Trial
| NCT Number | Sponsor | Condition | Start Date | Phase |
|---|---|---|---|---|
| NCT00869193 | Unilever R&D|University of Edinburgh |
Elevated Blood Pressure
|
March 2009 | Not Applicable |
| NCT01889368 | University of California, Davis|Illinois Institute of Technology |
Metabolic Syndrome
|
November 2012 | Not Applicable |
| NCT01190358 | University of California, Davis |
Metabolic Syndrome
|
August 2010 | Not Applicable |
| NCT03724578 | Cairo University |
Dental Caries
|
January 1, 2019 | Phase 1 |
| NCT00979732 | Clinical Nutrition Research Center, Illinois Institute of Technology|Polyphenolics, Inc. |
Hypertension
|
November 15, 2009 | Not Applicable |
| NCT05116150 | California Baptist University |
Dietary Supplementation
|
June 1, 2019 | Not Applicable |
| NCT00566553 | Mayo Clinic |
Breast Cancer
|
February 2008 | Not Applicable |
| NCT00713167 | Shahid Beheshti University of Medical Sciences|Tabriz University |
Hyperlipidemia
|
July 2008 | Phase 2|Phase 3 |
| NCT02939417 | Marwa Salem|Cairo University |
Retention & Fracture of Emax Laminate Veneers
|
November 2016 | Not Applicable |
| NCT04465110 | California Baptist University |
Obesity Adult Onset
|
September 1, 2019 | Not Applicable |
| NCT04136639 | Al-Azhar University |
Dental Caries
|
June 1, 2019 | Not Applicable |
| NCT01185067 | University of Michigan |
Diastolic Heart Failure|Hypertensive Heart Disease|Heart Failure With Preserved Ejection Fraction|Hypertension|Oxidative Stress
|
October 2010 | Phase 1 |
| NCT00554242 | Boston University |
Coronary Artery Disease
|
July 2007 | Not Applicable |
| NCT03087903 | University of Colorado, Denver |
Prostate Cancer
|
January 9, 2018 | Not Applicable |
| NCT04124016 | University of Parma|Azienda Ospedaliero-Universitaria di Parma |
Diet Modification
|
September 2, 2019 | Not Applicable |
| NCT04389060 | California Baptist University |
Obesity
|
March 1, 2019 | Not Applicable |
| NCT01190384 | University of California, Davis|Illinois Institute of Technology |
Metabolic Syndrome
|
August 2010 | Not Applicable |
| NCT01820299 | Medical University of South Carolina |
Solid Cancers
|
March 2013 | Phase 1 |
| NCT03030716 | Kansas State University|United States Department of Agriculture Foreign Agricultural Service |
Iron Deficiency Anemia|Iron-deficiency
|
June 25, 2016 | Not Applicable |
| NCT04357093 | Cairo University |
Dental Caries
|
February 2021 | Not Applicable |
Appearance
Solid
Shipping
Room temperature in continental US; may vary elsewhere.
Storage
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Solvent & Solubility
DMSO : 6 mg/mL (Need ultrasonic)
-
1.
-
2.
Add each solvent one by one: 10% DMSO >> 90% (20% SBE-β-CD in saline)
Solubility: ≥ 0.6 mg/mL (Infinity mM); Clear solution
References
- [1] The effects of grape seed extract (Vitis vinifera) supplement on inflammatory markers, neuropeptide Y, anthropometric measures, and appetite in obese or overweight individuals: A randomized clinical trial.
- [2] Inhibitory effects of grape seed extract on lipases. Nutrition. 2003 Oct;19(10):876-9.
- [3] Manjinder Kaur, et al. Grape seed extract inhibits in vitro and in vivo growth of human colorectal carcinoma cells. Clin Cancer Res. 2006 Oct 15;12(20 Pt 1):6194-202.
Synonyms