[CAS NO. 866893-90-5] Aftin-4
PRODUCTS SPECIFICATIONS [866893-90-5]
DESCRIPTION [866893-90-5]
Overview
| MDL | MFCD30187584 |
|---|---|
| Molecular Weight | 368.48 |
| Molecular Formula | C20H28N6O |
| SMILES | CC[C@@H](NC1=NC(N(C)CC2=CC=CC=C2)=C3N=CN(C(C)C)C3=N1)CO |
Summary
Aftin-4 is an Amyloid-β 42 ( Aβ 42 ) inducer.
IC50 & Target
Amyloid-β [1]
In Vitro
Aftin-4 selectively and potently increases Aβ 1-42 in N2a cells, primary neurons, and brain lysates, with an EC 50 value around 30 μM [1] .
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
In Vivo
Aftin-4 increases Aβ 1-42 levels in vivo in mice and provokes rapidly a sustained toxicity highly reminiscent of Alzheimer’s disease (AD). Aftin-4 is administered at increasing doses, between 3 and 20 nmol/mouse, into the lateral ventricle and animals are sacrificed at various time points, between 3 to 14 days after injection. The hippocampus is dissected out the contents in Aβ 1-40 or Aβ 1-42 is determined using a mouse ELISA assay. Aftin-4 dose-dependently and significantly increases Aβ 1-42 content, up to +216% at the highest dose tested [1] .
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Appearance
Solid
Shipping
Room temperature in continental US; may vary elsewhere.
Storage
| Powder | -20°C | 3 years |
|---|---|---|
| 4°C | 2 years | |
| In solvent | -80°C | 6 months |
| -20°C | 1 month |
Solvent & Solubility
DMSO : ≥ 100 mg/mL ( 271.39 mM )
* "≥" means soluble, but saturation unknown.
Stock Solutions
| Concentration Solvent Mass | 1 mg | 5 mg | 10 mg |
|---|
| 1 mM | 2.7139 mL | 13.5693 mL | 27.1385 mL |
| 5 mM | 0.5428 mL | 2.7139 mL | 5.4277 mL |
| 10 mM | 0.2714 mL | 1.3569 mL | 2.7139 mL |