[CAS NO. 890405-51-3] 3BDO

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PRODUCTS SPECIFICATIONS [890405-51-3]

Catalog

HY-U00434

Brand

MCE

CAS

890405-51-3

DESCRIPTION [890405-51-3]

Overview

MDL	MFCD30187363
Molecular Weight	327.33
Molecular Formula	C18H17NO5
SMILES	O=C1OC(COC2=C([N+]([O-])=O)C=CC=C2)CC1CC3=CC=CC=C3

For research use only. We do not sell to patients.

8 Publications Citing Use of MCE

Summary

3BDO is a new mTOR activator which can also inhibit autophagy .

IC50 & Target

mTOR

In Vitro

Phosphorylation of RPS6KB1 and EIF4EBP1 is significantly increased by 3BDO with vector alone but suppressed with FKBP1A overexpression. Rapamycin fails to decrease the phosphorylation of MTOR and RPS6KB1 in the presence of 3BDO. 3BDO suppresses the increase in MAP1LC3B puncta induced with rapamycin. 3BDO also inhibits the effect of rapamycin in HUVECs. The phosphorylation of Ser residues is decreased in HUVECs treated with 10 μM rapamycin, and 60 μM 3BDO reverses the phosphorylation. The results show that 3BDO suppresses the increased MAP1LC3B puncta number, MAP1LC3B-II level and decreased SQSTM1 protein level induced by rapamycin. 3BDO could dose- and time-dependently decrease FLJ11812 level in HUVECs. Overexpression of FLJ11812 reverses the inhibition of autophagy induced by 3BDO ^[1] .

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Immunofluorescence assay reveals that 3BDO treatment increases the level of p-p70S6K and decreases the protein level of ATG13 in plaque endothelium of mice. 3BDO does not affect the phosphorylation of mTOR direct downstream targets p70S6K and 4EBP1. As compare with controls, apoE ^-/- mice show inhibited endothelium autophagy and apoptosis with 3BDO treatment, so 3BDO protects against endothelium injury in atherosclerosis. 3BDO treatment stabilizes established atherosclerotic lesions in apoE ^-/- mice. In apoE ^-/- mice, as compare with controls, with 3BDO treatment, the serum level of IL-6 and IL-8 is significantly decreased ^[2] .

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Appearance

Solid-liquid mixture

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Pure form	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : ≥ 100 mg/mL ( 305.50 mM )

Ethanol : 20 mg/mL ( 61.10 mM ; Need ultrasonic)

* "≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.0550 mL	15.2751 mL	30.5502 mL
5 mM	0.6110 mL	3.0550 mL	6.1100 mL
10 mM	0.3055 mL	1.5275 mL	3.0550 mL

* Please refer to the solubility information to select the appropriate solvent.

In Vivo:

1.

Add each solvent one by one: 10% DMSO >> 40% PEG300 >> 5% Tween-80 >> 45% saline

Solubility: ≥ 2.5 mg/mL (7.64 mM); Clear solution
2.

Add each solvent one by one: 10% DMSO >> 90% (20% SBE-β-CD in saline)

Solubility: 2.5 mg/mL (7.64 mM); Suspended solution; Need ultrasonic
3.

Add each solvent one by one: 10% DMSO >> 90% corn oil

Solubility: ≥ 2.5 mg/mL (7.64 mM); Clear solution

* All of the co-solvents are available by MCE.