[CAS NO. 914805-33-7] Shield-1

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PRODUCTS SPECIFICATIONS [914805-33-7]

Catelog

HY-112210

Brand

MCE

CAS

914805-33-7

DESCRIPTION [914805-33-7]

Overview

MDL	-
Molecular Weight	748.90
Molecular Formula	C42H56N2O10
SMILES	O=C([C@H]1N(C([C@H](C2=CC(OC)=C(OC)C(OC)=C2)CC)=O)CCCC1)O[C@@H](C3=CC=CC(OCCN4CCOCC4)=C3)CCC5=CC=C(OC)C(OC)=C5

For research use only. We do not sell to patients.

Summary

Shield-1 (Shld1) is a specific, cell-permeant and high-affinity ligand of FK506-binding protein-12 ( FKBP ), and reverses the instability by binding to mutated FKBP (mtFKBP) , allowing conditional expression of mtFKBP-fused proteins. Shield-1 can stabilize proteins tagged with a mutated FKBP12-derived destabilization domain (DD) ^[1] ^[2] ^[3] .

In Vitro

Shield-1 (0.1 nM-1 μM) responses characterization of destabilizing domains ^[1] . Shield-1 (1 μM; 24 h) treatment shows excellent expression on both TRPV5 and YFP when fused the mtFKBP destabilizing domain to either TRPV5 or YFP, and leads to mtFKBP-TRPV5 forming a functional ion channel ^[2] .

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis ^[1]

Cell Line:	NIH3T3 cells
Concentration:	0.1 nM-1 μM
Incubation Time:
Result:	Stabilized the YFP fusion protein of L106P by higher concentrations of Shld1 (EC ₅₀ ∼100 nM).

Western Blot Analysis ^[2]

Cell Line:	HEK293 cells ^[2]
Concentration:	1 μM
Incubation Time:	24 hours
Result:	Expressed both TRPV5 and YFP well when Shield-1 in the medium, whereas in the abscence of Shield-1 decreased TRPV5 or YFP protein expression.

Appearance

Solid

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 67.5 mg/mL ( 90.13 mM ; ultrasonic and warming and heat to 60°C)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.3353 mL	6.6765 mL	13.3529 mL
5 mM	0.2671 mL	1.3353 mL	2.6706 mL
10 mM	0.1335 mL	0.6676 mL	1.3353 mL

* Please refer to the solubility information to select the appropriate solvent.

In Vivo:

1.

Add each solvent one by one: 10% DMSO >> 40% PEG300 >> 5% Tween-80 >> 45% saline

Solubility: ≥ 5.25 mg/mL (7.01 mM); Clear solution
2.

Add each solvent one by one: 10% DMSO >> 90% (20% SBE-β-CD in saline)

Solubility: 5.25 mg/mL (7.01 mM); Suspended solution; Need ultrasonic
3.

Add each solvent one by one: 10% DMSO >> 90% corn oil

Solubility: ≥ 5.25 mg/mL (7.01 mM); Clear solution

* All of the co-solvents are available by MCE.