[CAS NO. 959551-10-1] NIH-12848
PRODUCTS SPECIFICATIONS [959551-10-1]
DESCRIPTION [959551-10-1]
Overview
| MDL | MFCD30738011 |
|---|---|
| Molecular Weight | 385.41 |
| Molecular Formula | C20H14F3N3S |
| SMILES | FC(C1=CC=CC=C1C2=NC(NCC3=CC=CS3)=C4C=CC=CC4=N2)(F)F |
Summary
NIH-12848 is a putative phosphatidylinositol 5-phosphate 4-kinase γ ( PI5P4Kγ ) inhibitor with an IC 50 of 1 μM.
IC50 & Target
IC50: 1 μM (PI5P4Kγ) [1]
In Vitro
NIH-12848 inhibits PI5P4Kγ with an IC 50 of approximately 1 μM but does not inhibit the α and β PI5P4K isoforms at concentrations up to 100 μM. NIH-12848 inhibits the translocation of Na + / K + -ATPase to the plasma membrane that occurs when mpkCCD cells grow to confluence and also prevents reversibly their forming of ‘domes’ on the culture dish. Both these NIH-12848-induced effects are mimicked by specific RNAi knockdown of PI5P4Kγ, but not that of PI5P4Ks α or β. NIH-12848 could be a potentially powerful tool for exploring the cell physiology of PI5P4Ks [1] .
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Appearance
Solid
Shipping
Room temperature in continental US; may vary elsewhere.
Storage
| Powder | -20°C | 3 years |
|---|---|---|
| 4°C | 2 years | |
| In solvent | -80°C | 6 months |
| -20°C | 1 month |
Solvent & Solubility
DMSO : ≥ 100 mg/mL ( 259.46 mM )
* "≥" means soluble, but saturation unknown.
Stock Solutions
| Concentration Solvent Mass | 1 mg | 5 mg | 10 mg |
|---|
| 1 mM | 2.5946 mL | 12.9732 mL | 25.9464 mL |
| 5 mM | 0.5189 mL | 2.5946 mL | 5.1893 mL |
| 10 mM | 0.2595 mL | 1.2973 mL | 2.5946 mL |
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Add each solvent one by one: 10% DMSO >> 90% corn oil
Solubility: ≥ 2.5 mg/mL (6.49 mM); Clear solution