[CAS NO. 97-77-8]  Disulfiram

Disulfiram-copper complex potently inhibits the proteasomal activity in cultured breast cancer MDA-MB-231 and MCF10DCIS.com cells, but not normal, immortalized MCF-10A cells, before induction of apoptotic cancer cell death ^[1] . Disulfiram (DS), a clinically used anti-alcoholism drug, strongly inhibits constitutive and 5-FU-induced NF-kappaB activity in a dose-dependent manner. Disulfiram inhibits both NF-kappaB nuclear translocation and DNA binding activity but has no effect on 5-FU-induced IkappaBalpha degradation. Disulfiram significantly enhances the apoptotic effect of 5-FU on DLD-1 and RKO(WT) cell lines and synergistically potentiated the cytotoxicity of 5-FU to both cell lines. Disulfiram also effectively abolishes 5-FU chemoresistance in a 5-FU resistant cell line H630(5-FU) in vitro ^[2] . Oseltamivir decreases the number of viable cells, and the addition of CuCl ₂ significantly enhances the DSF-induced cell death to less than 10% of control ^[3] . Disulfiram given to melanoma cells in combination with Cu ²⁺ or Zn ²⁺ decreases expression of cyclin A and reduces proliferation in vitro at lower concentrations than disulfiram alone ^[4] .
Disulfiram (0.1 nM-10 μM; 72 h) + Cu ²⁺ combination enhances the cytotoxicity on ovarian cancer cell lines ^[1] .

MCE has not independently confirmed the accuracy of these methods. They are for reference only.