[CAS NO. 99217-63-7] Kushenol A
PRODUCTS SPECIFICATIONS [99217-63-7]
DESCRIPTION [99217-63-7]
Overview
| MDL | - |
|---|---|
| Molecular Weight | 408.49 |
| Molecular Formula | C25H28O5 |
| SMILES | O=C1C[C@@H](C2=CC=CC=C2O)OC3=C(C[C@H](C(C)=C)C/C=C(C)\C)C(O)=CC(O)=C13 |
1 Publications Citing Use of MCE
Summary
Kushenol A (Leachianone E) is isolated from the root of Sophora flavescent . Kushenol A is a non-competitive tyrosinase inhibitor to block the conversion of L-tyrosine to L-DOPA, shows IC 50 and K i values of 1.1 μM and 0.4 μM, respectively [1] . Kushenol A is a flavonoid antioxidant, has inhibitory effects on alpha-glucosidase ( IC 50 : 45 μM; K i : 6.8 μM) and β-amylase [2] . Kushenol A is confirmed as potential inhibitors of enzymes targeted by cosmetics for skin whitening and aging [1] .
In Vitro
Kushenol A (25 μM) exhibits a highly potent inhibitory activity on ABTS+ radical scavenging with an IC
50
value of 9.7 ± 0.1 μM, and exhibits inhibits scavenging activity as a percentage 93.7% at 25 μM
[1]
.
Kushenol A (0-60 μg/ml; 24 hours) shows considerable cytotoxic effects against NSCLC cells, exhibits IC
50
values of 5.3 μg/ml and 20.5 μg/ml for A549 and NCI‐H226 cells, respectively. It shows an IC
50
value of 57.2 μg/ml for BEAS-2B cells
[3]
.
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Cell Viability Assay [3]
| Cell Line: |
NSCLC cell lines, A549 and NCI-H226
Normal human lung epithelial: BEAS-2B |
| Concentration: | 0-60 μg/ml |
| Incubation Time: | 24 hours |
| Result: | Exhibited considerable cytotoxic effects against NSCLC cells. |
Appearance
Solid
Source
Shipping
Room temperature in continental US; may vary elsewhere.
Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvent & Solubility
DMSO : 100 mg/mL ( 244.80 mM ; Need ultrasonic)
Stock Solutions
| Concentration Solvent Mass | 1 mg | 5 mg | 10 mg |
|---|
| 1 mM | 2.4480 mL | 12.2402 mL | 24.4804 mL |
| 5 mM | 0.4896 mL | 2.4480 mL | 4.8961 mL |
| 10 mM | 0.2448 mL | 1.2240 mL | 2.4480 mL |
References
- [1] Kim JH, et al. Kushenol A and 8-prenylkaempferol, tyrosinase inhibitors, derived from Sophora flavescens. J Enzyme Inhib Med Chem. 2018 Dec;33(1):1048-1054.
- [2] Kim JH, et al. Glycosidase inhibitory flavonoids from Sophora flavescens. Biol Pharm Bull. 2006 Feb;29(2):302-5.
- [3] Chen H, et al. A Novel Flavonoid Kushenol Z from Sophora flavescens Mediates mTOR Pathway by Inhibiting Phosphodiesterase and Akt Activity to Induce Apoptosis in Non-Small-Cell Lung Cancer Cells.Molecules. 2019 Dec 4;24(24). pii: E4425.
Synonyms