Applications
Detect mouse/rabbit primary Ab on human tissue
Product Features
• Higher Sensitivity – Delivers greater sensitivity compared to the MicroStacker™ Universal system, ensuring improved detection of target antigens
• Dual Compatibility – Detects both mouse and rabbit primary antibodies on human tissue, providing versatility across a wide range of applications
• Two-Step Method – Utilizes a manual two-step process with a post-primary linker, enhancing signal intensity and detection accuracy
• Enhanced Detection – Increased sensitivity through the addition of a post-primary linker, resulting in clearer and more defined staining
Recommended Protocol
• Primary Antibody: Room temperature, 30 minutes
• Post-Primary Linker: Room temperature, 30 minutes
• Detection Polymer: Room temperature, 30 minutes
• DAB Incubation: 5 minutes
Application Notes
The MicroStacker™ Polymer Detection System (Enhanced) provides the best overall performance among manual detection systems, offering unmatched sensitivity and clarity for challenging immunohistochemical applications.
MicroStacker™ Technology Platform
The Enhanced MicroStacker™ Polymer Detection System is designed to surpass the performance of standard universal HRP polymers by incorporating a two-step detection process. This system detects both mouse and rabbit primary antibodies on human tissue with significantly heightened sensitivity, achieved through the use of a post-primary linker.
Celnovte’s proprietary MicroStacker™ technology ensures Fab’ fragments are directionally conjugated to the poly-HRP core using micro-polymer scaffolds. This advanced conjugation process prevents the blocking of antibody binding sites, maintaining high sensitivity and consistent results.
The compact polymer structure enables deep tissue penetration, providing uniform and reproducible staining of nuclear, cytoplasmic, and membranous antigens. By eliminating endogenous biotin interference and reducing non-specific Fc receptor binding through Fab’ fragments, this system achieves exceptional specificity and minimal background staining, making it ideal for high-demand IHC applications.
