Applications
Detection of Mouse Primary Antibodies on Human Tissue in IHC
Storage Conditions
2-8 C | 36-46 F
Technology
Micropolymer with Fab' Fragments
Format
Ready-to-Use
Product Features
• Original MicroStacker™ Technology – Proprietary technology for accurate detection of mouse primary antibodies on human tissue
• Specific Detection – Tailored for detecting mouse primary antibodies with high specificity and minimal background interference
• One-Step Method – Streamlined manual workflow with a single-step detection process for ease of use
• Superior Sensitivity – Provides enhanced sensitivity and lower background compared to conventional bulky dextran-based HRP polymers
Recommended Protocol
• Primary Antibody: Room temperature, 30-60 minutes
• Detection Polymer: Room temperature, 30 minutes
• DAB Incubation: 5 minutes
MicroStacker™ Technology Platform
MicroStacker™ Goat Anti-Mouse HRP Polymer is a biotin-free, polymeric horseradish peroxidase (HRP) secondary antibody system designed for detecting mouse primary antibodies on human tissue. This advanced detection system leverages Celnovte’s proprietary MicroStacker™ technology, which significantly enhances sensitivity and reduces background, outperforming traditional dextran-based HRP polymers.
Through the use of micro-polymer scaffolds, Fab’ fragments are directionally conjugated to the poly-HRP core, ensuring that antibody binding sites remain unblocked during conjugation. This improves the polymer’s sensitivity, resulting in clearer, more consistent staining across tissue samples.
The compact polymer structure allows deep tissue penetration, enabling uniform staining of nuclear, cytoplasmic, and membranous antigens. By eliminating biotin from the detection process, the system avoids background staining caused by endogenous biotin. Additionally, the use of Fab’ fragments instead of whole IgG reduces non-specific Fc receptor binding, ensuring precise and highly specific results suitable for a variety of immunohistochemical applications.
