Applications
Detection of Rabbit Primary Antibodies on Human Tissue in IHC
Storage Conditions
2-8 C | 36-46 F
Technology
Micropolymer with Fab' Fragments
Format
Ready-to-Use
Product Features
• Original MicroStacker™ Technology – Proprietary technology for precise detection of rabbit primary antibodies on human tissue
• Specific Detection – Designed for the detection of rabbit primary antibodies, ensuring high specificity and sensitivity
• One-Step Method – Simplified manual workflow with a streamlined, single-step detection process
• Superior Sensitivity – Delivers higher sensitivity and reduced background compared to conventional HRP polymers with bulky dextran backbones
Recommended Protocol
• Primary Antibody: Room temperature, 30-60 minutes
• Detection Polymer: Room temperature, 30 minutes
• DAB Incubation: 5 minutes
MicroStacker™ Technology Platform
MicroStacker™ Goat Anti-Rabbit HRP Polymer is a biotin-free, polymeric horseradish peroxidase (HRP) secondary antibody system designed for the detection of rabbit primary antibodies on human tissue. This system utilizes advanced MicroStacker™ technology to ensure high sensitivity and minimal background, outperforming traditional bulky dextran-based HRP polymers.
Celnovte’s proprietary MicroStacker™ technology employs micro-polymer scaffolds to facilitate the directional attachment of Fab’ fragments to the poly-HRP core. This innovative approach prevents the blocking of antibody binding sites during conjugation, enhancing the polymer’s sensitivity and ensuring consistent and robust staining.
The compact polymer structure penetrates deeply into cellular compartments, providing uniform staining across nuclear, cytoplasmic, and membranous antigens. The biotin-free nature of the system eliminates background caused by endogenous biotin, while the use of Fab’ fragments minimizes non-specific Fc receptor binding. The result is a detection system that delivers superior clarity, specificity, and sensitivity, ideal for a wide range of immunohistochemical applications.
