Applications
Detection of Mouse/Rabbit Primary Antibodies on Human Tissue in IHC
Storage Conditions
2-8 C | 36-46 F
Technology
Micropolymer with Fab' Fragments
Format
Ready-to-Use
Product Features
• Original MicroStacker™ Technology – Proprietary technology enabling high-sensitivity detection of mouse and rabbit primary antibodies on human tissue
• Universal Detection – Compatible with both mouse and rabbit primary antibodies, offering broad application across human tissue samples
• One-Step Method – Simplified manual workflow with a single-step detection process
• Superior Sensitivity – Outperforms conventional HRP polymers with bulky dextran backbones, providing clearer and more precise staining results
Recommended Protocol
• Primary Antibody: Room temperature, 30-60 minutes
• Detection Polymer: Room temperature, 30 minutes
• DAB Incubation: 5 minutes
MicroStacker™ Technology Platform
MicroStacker™ RX HRP Polymer is a universal, biotin-free, polymeric horseradish peroxidase (HRP) secondary antibody system designed to detect both mouse and rabbit primary antibodies on human tissue. This versatile detection polymer is engineered to deliver exceptional sensitivity and reduce background noise, surpassing conventional bulky dextran-based HRP polymers.
Utilizing Celnovte’s proprietary MicroStacker™ technology, Fab’ fragments are directionally conjugated to the poly-HRP core through micro-polymer scaffolds. This approach prevents the obstruction of antibody binding sites during the conjugation process, enhancing sensitivity and ensuring consistent signal intensity.
The compact polymeric structure allows deep penetration into cellular compartments, providing uniform staining across nuclear, cytoplasmic, and membranous antigens. By eliminating the use of biotin, the system avoids endogenous biotin-related background staining. Additionally, the use of Fab’ fragments reduces non-specific Fc receptor binding, resulting in highly specific and reliable results suitable for a wide range of applications.
