Applications
Detection of Mouse Primary Antibodies on Mouse Tissue in IHC
Storage Conditions
2-8 C | 36-46 F
Technology
Micropolymer with Fab' Fragments
Format
Ready-to-Use
Product Features
• Original MicroStacker™ Technology – Utilizes proprietary technology for superior detection of mouse primary antibodies on mouse tissue
• Special Treatment – Reduces reactivity with endogenous mouse IgG, minimizing background interference
• No Blocking Required – Eliminates the need for endogenous mouse IgG blocking steps, simplifying protocols
• Reduced Background – Provides lower background and clearer staining compared to conventional Mouse-on-Mouse methods
Recommended Protocol
• Primary Antibody: Room temperature, 30-60 minutes
• Detection Polymer: Room temperature, 12 minutes
• DAB Incubation: 5 minutes
MicroStacker™ Technology Platform
MicroStacker™ Mouse-on-Mouse Polymer is a biotin-free, polymeric horseradish peroxidase (HRP) secondary antibody conjugate system designed to detect mouse primary antibodies on mouse tissue. This system features specially engineered anti-mouse secondary antibodies to minimize high background caused by endogenous mouse IgG.
Celnovte’s proprietary MicroStacker™ technology uses micro-polymer scaffolds to enable orientation-controlled attachment of Fab’ fragments to the poly-HRP core. This proprietary Fab’ labeling method prevents the antibody binding site from being blocked during bioconjugation, enhancing the polymer’s sensitivity. The compact polymeric structure penetrates all cellular compartments, ensuring consistent staining of nuclear, cytoplasmic, and membranous antigens.
The biotin-free detection system prevents background staining from endogenous biotin. Additionally, the use of Fab’ fragments instead of whole IgG reduces non-specific binding to endogenous Fc receptors, further minimizing background. This results in superior sensitivity and specificity compared to traditional HRP polymers with bulky dextran backbones.
