Applications
Detection of Rabbit Primary Antibodies on Mouse/Rat Tissue in IHC
Storage Conditions
2-8 C | 36-46 F
Technology
Micropolymer with Fab' Fragments
Format
Ready-to-Use
Product Features
• Original MicroStacker™ Technology – Advanced technology designed to detect rabbit primary antibodies on mouse and rat tissue
• Cross-Adsorbed – Cross-adsorbed against mouse and rat IgG to minimize background staining caused by endogenous IgG
• Specific Detection – Reduces non-specific binding, ensuring clearer and more precise staining results compared to non-adsorbed detection systems
• Enhanced Clarity – Delivers lower background and sharper staining, providing superior results in rodent tissue applications
Recommended Protocol
• Primary Antibody: Room temperature, 30-60 minutes
• Detection Polymer: Room temperature, 30 minutes
• DAB Incubation: 5 minutes
MicroStacker™ Technology Platform
MicroStacker™ Rabbit-on-Rodent HRP Polymer is a biotin-free, polymeric horseradish peroxidase (HRP) secondary antibody system engineered for the detection of rabbit primary antibodies on mouse and rat tissue. This system is cross-adsorbed against mouse and rat IgG to reduce non-specific binding and background staining from endogenous IgG, significantly enhancing staining clarity and sensitivity.
Celnovte’s proprietary MicroStacker™ technology utilizes micro-polymer scaffolds to facilitate the directional attachment of Fab’ fragments to the poly-HRP core. This directional conjugation prevents the blocking of antibody binding sites, improving sensitivity and ensuring consistent, high-quality staining.
The compact polymeric structure enables deep penetration into tissue, providing uniform staining across nuclear, cytoplasmic, and membranous antigens. By eliminating biotin from the detection process, the system prevents background staining associated with endogenous biotin. Additionally, the use of Fab’ fragments minimizes non-specific Fc receptor binding, resulting in highly specific and reproducible results for rodent tissue applications.
