Applications
Detection of Rabbit Primary Antibodies on Human Tissue in IHC
Storage Conditions
2-8 C | 36-46 F
Technology
Micropolymer with Fab' Fragments
Format
Ready-to-Use
Product Features
• 1-Step System – Efficient manual detection system utilizing proprietary MicroStacker™ technology for streamlined workflows
• Specific Detection – Accurately detects rabbit primary antibodies on human tissue with high sensitivity
• Bright, High-Contrast Signal – Fast Red substrate generates a vivid signal, ensuring strong contrast against background for clear visualization
• Rapid and Reliable – Provides fast and consistent staining, outperforming conventional AP polymers
Recommended Protocol
• Primary Antibody: Room temperature, 30-60 minutes
• Detection Polymer: Room temperature, 30 minutes
• Fast Red Chromogen: 6-8 minutes
Application Notes
• Ideal for Pigmented Tissues – Suitable for tissues with endogenous pigments, such as melanoma and lung cancer, ensuring clear staining and visualization
• Multiplex Compatibility – Compatible with sequential multiplex IHC applications, enabling detection of multiple targets on the same tissue section without cross-reactivity
MicroStacker™ Technology Platform
The MicroStacker™ Goat Anti-Rabbit AP Polymer is a biotin-free, alkaline phosphatase (AP) polymer system designed to detect rabbit primary antibodies on human tissue. This advanced detection system uses Fast Red chromogen to deliver bright, high-contrast staining, making it ideal for tissues with high pigment levels.
Leveraging Celnovte’s proprietary MicroStacker™ technology, the system utilizes micro-polymer scaffolds that directionally attach Fab’ fragments to the poly-AP core. This innovative approach prevents antibody binding site blockage during conjugation, enhancing sensitivity and reducing background interference.
The compact polymeric structure penetrates tissue efficiently, providing uniform staining across nuclear, cytoplasmic, and membranous antigens. By eliminating endogenous biotin interference and reducing non-specific Fc receptor binding, the system achieves outstanding specificity and reproducibility. This makes it a preferred solution for complex immunohistochemical applications and sequential multiplex IHC workflows.
