[CAS NO. 2470015-35-9] Anticancer agent 43
PRODUCTS SPECIFICATIONS [2470015-35-9]
DESCRIPTION [2470015-35-9]
Overview
| MDL | - |
|---|---|
| Molecular Weight | 336.36 |
| Molecular Formula | C14H9FN2O3S2 |
| SMILES | COC(C1=C(C2=C(N1)C=CC(F)=C2)/C=C3SC(NC\3=O)=S)=O |
Summary
Anticancer Agent 43 is a potent anticancer agent. Anticancer Agent 43 induces apoptosis by caspase 3 , PARP1 , and Bax dependent mechanisms. Anticancer Agent 43 induces DNA damage [1] .
IC50 & Target
|
Caspase 3 |
PARP1 |
Bax |
In Vitro
Anticancer
agent 43 (compound 3a) shows selectivity toward human tumor cells (SI
50
=28.94)
[1]
.
Anticancer
agent 43 (45 µM, 24 h) induces
apoptosis
via
caspase 3
,
PARP1
and
Bax
dependent pathways in HepG2 cells
[1]
.
Anticancer
agent 43 (45 µM, 24 h) shows no effect on the transition of G1/S phases in HepG2 cells
[1]
.
Anticancer
agent 43 (0.7, 45, 55 µM) induces DNA damage in HCT116 cells (Tail DNA=16.1%, OTM=3.7), MCF-7 cells, HepG2 cells (Tail DNA=26.2%, OTM=13.2), Balb/c 3T3 cells (Tail DNA = 8.4%, OTM = 3.5)
[1]
.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Cell Cytotoxicity Assay [1]
| Cell Line: | HepG2, MCF-7, HCT116, HeLa, A549, WM793, THP-1, HaCaT, Balb/c3T3 cells |
| Concentration: | 0, 1, 10, 100 µM |
| Incubation Time: | 72 h |
| Result: | Showed cytotoxic action with GI 50 s of 12.1, 0.7, 0.8, 49.3, 9.7 µM for for HepG2, MCF-7, HCT116, HeLa, A549 cells, low toxicity towards WM793, THP-1, HaCaT, Balb/c 3T3 cells with GI 50 s of 80.4, 62.4,98.3,40.8 µM , respectively. |
Apoptosis Analysis [1]
| Cell Line: | HepG2 cells |
| Concentration: | 45 µM |
| Incubation Time: | 24 h |
| Result: | Induced apoptosis in HepG2 cells via caspase 3, PARP1 and Bax dependent pathways. |
Western Blot Analysis [1]
| Cell Line: | HCT116, MCF-7 cells |
| Concentration: | 0.7 µM |
| Incubation Time: | 24 h |
| Result: | Decreased the expression of Cdk2 protein in HCT116 and MCF-7 cells. |
Cell Cycle Analysis [1]
| Cell Line: | HepG2 cells |
| Concentration: | 45 µM |
| Incubation Time: | 24 h |
| Result: | Showed no effect on the transition of G1/S phases in HepG2 cells. |
Appearance
Solid
Shipping
Room temperature in continental US; may vary elsewhere.
Storage
| Powder | -20°C | 3 years |
|---|---|---|
| 4°C | 2 years | |
| In solvent | -80°C | 6 months |
| -20°C | 1 month |
Solvent & Solubility
DMSO : 125 mg/mL ( 371.63 mM ; Need ultrasonic)
Stock Solutions
| Concentration Solvent Mass | 1 mg | 5 mg | 10 mg |
|---|
| 1 mM | 2.9730 mL | 14.8650 mL | 29.7301 mL |
| 5 mM | 0.5946 mL | 2.9730 mL | 5.9460 mL |
| 10 mM | 0.2973 mL | 1.4865 mL | 2.9730 mL |