[CAS NO. 3032997-16-0] CDK1-IN-4
PRODUCTS SPECIFICATIONS [3032997-16-0]
DESCRIPTION [3032997-16-0]
Overview
| MDL | - |
|---|---|
| Molecular Weight | 474.02 |
| Molecular Formula | C26H24ClN5S |
| SMILES | S=C(N1CCN(C2=NN=C(CC3=CC=CC=C3)C4=C2C=CC=C4)CC1)NC5=CC=CC(Cl)=C5 |
Summary
IC50 & Target
|
CDK1 44.52 nM (IC 50 ) |
CDK2 621.93 nM (IC 50 ) |
CDK5 135.22 nM (IC 50 ) |
In Vitro
CDK1-IN-4 (0-10 μM; 24 h) inhibits the growth of PDAC, melanoma, leukemia, colon and breast cancer cells
[1]
.
CDK1-IN-4 (0-1 μM) inhibits CDK1, CDK2 and CDK5 with IC
50
s of 36.8, 305.17 and 369.37 nM, respectively
[1]
.
CDK1-IN-4 (0-10 μM) inhibits AXL, PTK2B, FGFR, JAK1, IGF1R and BRAF kinases with IC
50
s of 2488, 8957, 7620, 8541, 4294 and 1156 nM, respectively
[1]
.
CDK1-IN-4 (0.88 μM; 24 h) decreases CDK1 protein level in vitro and affects cell cycle
[1]
.
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Cell Proliferation Assay [1]
| Cell Line: | PDAC, melanoma, leukemia, colon and breast cancer cell lines |
| Concentration: | 0-10 μM |
| Incubation Time: | 24 hours |
| Result: | Inhibited cell growth of PDAC, melanoma, leukemia, colon, and breast cancer cell over 69%, and inhibited MDA-PATC53 and PL45 cells with IC 50 s of 0.88 and 1.14 μM, respectively. |
Western Blot Analysis [1]
| Cell Line: | MDA-PATC53 cell line |
| Concentration: | 0.88 μM |
| Incubation Time: | 24 hours |
| Result: | Downregulated CDK1 protein level compared to untreated cells. |
Cell Cycle Analysis [1]
| Cell Line: | MDA-PATC53 cell line |
| Concentration: | 0.88 μM |
| Incubation Time: | 24 hours |
| Result: | Significantly arrested in G2/M phase of the cell cycle compared with the untreated cells. |
Shipping
Room temperature in continental US; may vary elsewhere.
Storage
Please store the product under the recommended conditions in the Certificate of Analysis.