[CAS NO. 3032997-20-6] CDK1-IN-5
PRODUCTS SPECIFICATIONS [3032997-20-6]
DESCRIPTION [3032997-20-6]
Overview
| MDL | - |
|---|---|
| Molecular Weight | 504.05 |
| Molecular Formula | C27H26ClN5OS |
| SMILES | S=C(N1CCN(C2=NN=C(CC3=CC=C(OC)C=C3)C4=C2C=CC=C4)CC1)NC5=CC=CC(Cl)=C5 |
Summary
IC50 & Target
|
CDK1 42.19 nM (IC 50 ) |
CDK2 188.71 nM (IC 50 ) |
CDK5 354.15 nM (IC 50 ) |
In Vitro
CDK1-IN-5 (0-10 μM; 24 h) inhibits the growth of various cancer cells
[1]
.
CDK1-IN-5 (0-1 μM) inhibits CDK1, CDK2 and CDK5 with IC
50
s of 42.19, 188.71 and 354.15 nM, respectively
[1]
.
CDK1-IN-5 (0-10 μM) inhibits AXL, PTK2B, FGFR, JAK1, IGF1R and BRAF kinases with IC
50
s of 5649, 8945, 2538, 2417, 8546 and 8138 nM, respectively
[1]
.
CDK1-IN-5 (0.73 μM; 24 h) decreases CDK1 protein level in virto and affects cell cycle
[1]
.
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Cell Proliferation Assay [1]
| Cell Line: | PDAC, melanoma, leukemia, colon, and breast cancer cell lines |
| Concentration: | 0-10 μM |
| Incubation Time: | 24 hours |
| Result: | Inhibited cell growth of PDAC, melanoma, leukemia, colon and breast cancer cells over 62%, and inhibited MDA-PATC53 and PL45 cells with IC 50 s of 0.73 and 1 μM, respectively. |
Western Blot Analysis [1]
| Cell Line: | MDA-PATC53 cell line |
| Concentration: | 0.73 μM |
| Incubation Time: | 24 hours |
| Result: | Downregulated the CDK1 protein level compared to untreated cells. |
Cell Cycle Analysis [1]
| Cell Line: | MDA-PATC53 cell line |
| Concentration: | 0.73 μM |
| Incubation Time: | 24 hours |
| Result: | Significantly arrested in G2/M phase of the cell cycle compared with the untreated cells. |
Shipping
Room temperature in continental US; may vary elsewhere.
Storage
Please store the product under the recommended conditions in the Certificate of Analysis.