[CAS NO. ] Calcitonin Gene Related Peptide (CGRP) II, rat TFA

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Catalog:	HY-P1913A
Brand:	MCE
CAS:	-

Overview

MDL	-
Molecular Weight	3919.33
Molecular Formula	C165H268F3N51O52S2
SMILES	-

For research use only. We do not sell to patients.

Summary

Calcitonin Gene Related Peptide (CGRP) II, rat TFA, a CGRP receptor activator, is a potent and long-lasting vasodilator . Calcitonin Gene Related Peptide (CGRP) II TFA can be used in the research of cardiovascular diseases ^[1] .

In Vitro

Calcitonin Gene Related Peptide (CGRP) II, rat TFA induces vasodilator effects in small-diameter pig left anterior descending (LAD) coronary arteries with an EC ₅₀ value of 0.56 nM ^[1] .
Calcitonin Gene Related Peptide (CGRP) II, rat TFA relaxes the spontaneous tone in isolated internal anal sphincter (IAS) strips, with an EC ₅₀ value of 83 μM ^[2] .
Calcitonin Gene Related Peptide (CGRP) II, rat TFA (rβCGRP) inhibits [ ¹²⁵ I]hαCGRP binding (IC ₅₀ : 7 nM), and stimulates cAMP accumulation (EC ₅₀ : 0.56 nM) in the cells co-expressing mRAMP1 (mouse receptor-activity-modifying-protein 1) and rCRLR (rat calcitonin receptor-like receptor) ^[3] .
Calcitonin Gene Related Peptide (CGRP) II, rat TFA (rat-βCGRP, 0.01-100 nM) induces endothelium-independent relaxations in the coronary arteries from male and female Sprague-Dawley rats, with an IC ₅₀ value of 2.8 nM approximately ^[4] .

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Calcitonin Gene Related Peptide (CGRP) II, rat TFA (rat-βCGRP, bolus injection, 0.3 μg/kg, at 10 min intervals) induces hypotension and vasodilatation in Male Sprague-Dawley rats ^[5] .

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Male Sprague-Dawley rats ^[5]
Dosage:	0.01 ng/kg-3 μg/kg (100 μL), succeeded by a 150 μL isotonic saline flush, at 10 min intervals.
Administration:	A bolus injection
Result:	Induced PA (pial artery) dilatation and increased in LCBF _Flux (local cortical cerebral blood flow).

Appearance

Solid

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Sealed storage, away from moisture

Powder	-80°C	2 years
	-20°C	1 year

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

H ₂ O

Peptide Solubility and Storage Guidelines:

1. Calculate the length of the peptide.

2. Calculate the overall charge of the entire peptide according to the following table:

	Contents	Assign value
Acidic amino acid	Asp (D), Glu (E), and the C-terminal -COOH.	-1
Basic amino acid	Arg (R), Lys (K), His (H), and the N-terminal -NH ₂	+1
Neutral amino acid	Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q)	0

3. Recommended solution:

Overall charge of peptide	Details
Negative (<0)	1. Try to dissolve the peptide in water first. 2. If water fails, add NH ₄ OH (<50 μL). 3. If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
Positive (>0)	1. Try to dissolve the peptide in water first. 2. If water fails, try dissolving the peptide in a 10%-30% acetic acid solution. 3. If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
Zero (=0)	1. Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first. 2. For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.

References

[1] D Wu, et al. Development and potential of non-peptide antagonists for calcitonin-gene-related peptide (CGRP) receptors: evidence for CGRP receptor heterogeneity. Biochem Soc Trans. 2002 Aug;30(4):468-73.
[2] F M Wisskirchen, et al. CGRP(2) receptor in the internal anal sphincter of the rat: implications for CGRP receptor classification. Br J Pharmacol. 2000 May;130(2):464-70.
[3] K Husmann, et al. Mouse receptor-activity-modifying proteins 1, -2 and -3: amino acid sequence, expression and function. Mol Cell Endocrinol. 2000 Apr 25;162(1-2):35-43.
[4] M Sheykhzade, et al. Characterization of calcitonin gene-related peptide (CGRP) receptors in intramural coronary arteries from male and female Sprague Dawley rats. Br J Pharmacol. 1998 Apr;123(7):1464-70.
[5] Kenneth A Petersen, et al. Inhibitory effect of BIBN4096BS on cephalic vasodilatation induced by CGRP or transcranial electrical stimulation in the rat. Br J Pharmacol. 2004 Nov;143(6):697-704.

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