[CAS NO. 30516-87-1]  Zidovudine

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PRODUCTS SPECIFICATIONS [30516-87-1]

Catelog
SLK-S2579
Brand
Selleck
CAS
30516-87-1

DESCRIPTION [30516-87-1]

Overview

MDL-
Molecular Weight267.24
Molecular FormulaC10H13N5O4
SMILESO=C1N([C@]2(C[C@H](N=[N+]=[N-])[C@@H](CO)O2)[H])C=C(C)C(=O)N1

For research use only.

Storage

3 years,-20°C,powder
1 years,-80°C,in solvent

Shipping

Room temperature shipping(Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

1 mg5 mg10 mg
1 mM3.7420 mL18.7098 mL37.4195 mL
5 mM0.7484 mL3.7420 mL7.4839 mL
10 mM0.3742 mL1.8710 mL3.7420 mL
50 mM0.0748 mL0.3742 mL0.7484 mL

Description

Zidovudine (ZDV, Azidothymidine, NSC 602670,Retrovir) is a nucleoside analogue inhibitor, used to treat HIV. It could decrease the HDR efficiency and decrease CRISPR-mediated sequence-specific genome knockin events while increaseing knockout efficiency.

Targets

Reverse transcriptase [1]

In vitro

Zidovudine pretreatment has potent anti-HIV-1 activity in the newly infected T and monocytic cells but not in chronically infected cells. Inhibition of reverse transcription by Zidovudine decreases p24 antigen levels modestly, decreased HIV-1 gag by 19-fold, and inhibits detection of 2-LTR HIV-1 DNA. Zidovudine and dideoxynucleosides deplete wild-type mitochondrial DNA levels and increase deletedmitochondrial DNA levels in cultured Kearns-Sayre syndrome fibroblasts. Zidovudine (AZT, 0.1-50 mM) has a concentration dependent suppressive effect on the growth of granulocyte-monocyte colony forming unit (CFU-GM) derived colonies. Zidovudine exposure also induces a concentration dependent suppressive effect (35-90%) on GM-CSF receptor type alpha (GM-CSFR alpha) gene expression. Zidovudine causes a much lower decrease (15-22%) on the IL-3 receptor type alpha (IL-3R alpha) message level, and has an insignificant effect on glyceraldehyde 3-phosphate dehydrogenase (GAPDH) and c-myc message levels. Zidovudine causes a concentration-dependent inhibition in the levels of the mRNA of Epo receptors and c-fos, whereas the level of c-myc mRNA is unaffected. Zidovudine also inhibits protein kinase C (PKC) activity in a concentration- and time-dependent manner, causing 50% inhibition at 10 mM within 3 hours. Zidovudine-induced down-regulation of Epo receptors and c-fos expression coupled with inhibition of Epo receptor-mediated signal transduction through PKC are significant contributory factors to AZT-induced erythroid toxicity. Zidovudine could decrease the HDR efficiency. It decrease CRISPR-mediated sequence-specific genome knockin events while increases knockout efficiency .


Synonyms

Thymidine, 3′-azido-3′-deoxy-
3′-Azido-3′-deoxythymidine
Azidothymidine
3′-Deoxy-3′-azidothymidine
AZT
BW-A 509U
AZT (pharmaceutical)
Zidovudine
Retrovir
3′-Azidothymidine
NSC 602670
Azitidin
ZDV
Timazid
Retrovir IV
ZVD
3-Azido-3-deoxythymidine
Compound S
Viro-Z
Zido-H
Retrovis
3′-AZT