IWP-L6 blocks the phosphorylation of the cytoplasmic Wnt pathway effector Dvl2 in HEK293 cells. In cultured embryonic kidneys, IWP-L6 also inhibits Wnt mediated branching morphogenesis by reversibly blocking Wnt signaling.
In vivo
IWP-L6 is stable in human plasma over 24 h, while rapidly metabolized in rat plasma (t1/2 = 190 min), murine plasma (t1/2 = 2 min), and the murine liver S9 fractions. In embryonic zebrafish, IWP-L6 effectively inhibits posterior axis formation, a Wnt/β-catenin dependent developmental process.
